Journal: Cell Reports

Article Title: A cell-type-specific alternative splicing regulator shapes synapse properties in a trans -synaptic manner

doi: 10.1016/j.celrep.2023.112173

Figure Lengend Snippet:

Article Snippet: Additional antibodies are rat anti-HA (Roche, #11867431001, 1:1000), mouse anti-NeuN (Chemicon #MAB377 1:2000), and rabbit anti-CTIP2 (Novus Biologicals, #NB100-2600).

Techniques: Recombinant, Magnetic Beads, Concentration Assay, Reverse Transcription, Western Blot, Fluorescence, Software

Journal: Molecular Neurobiology

Article Title: Deletion of TRPC6 , an Autism Risk Gene, Induces Hyperexcitability in Cortical Neurons Derived from Human Pluripotent Stem Cells

doi: 10.1007/s12035-023-03527-0

Figure Lengend Snippet: Characterization of human cortical neurons generated in vitro from pluripotent stem cells. a Schematic of hPSC differentiation into NPCs and mature cortical neurons. The arrows indicate the time point of splitting the cells. b Immunostaining of control and TRPC6 KO cortical neurons (from two different hPSC clones, C21 and C47) differentiated for 8 weeks. Cortical neuron markers; CTIP2, SATB2, TBR2, and BRN2. MAP2, BTUB; pan-neuronal markers. c GFAP, a marker for glia. vGLUT1, a marker for glutamatergic excitatory synapses. d Syn, a synaptic vesicle protein. Nuclei are stained with DAPI. e Validation of TRPC6 protein expression in TRPC6 KO cortical neurons differentiated for 8 weeks. Scale, 100 µm

Article Snippet: Primary antibodies consisted of SOX2 (Rabbit, 1:200, Invitrogen: MA1-014), FOXG1 (Rabbit, 1:200, Abcam: ab18259), OTX2 (Goat, 1:300, R&D: AF1979), Nestin (Mouse, 1:100, Invitrogen: MA1110, MAP2 (Chicken, 1:500, Abcam: ab5392), MAP2 (Mouse, 1:500, Invitrogen: 13–1500), beta-tubulin III (Mouse, 1:300, MAB1637), TBR2 (Rabbit,1:300, Cell signaling: 66325), CTIP2 (Rabbit, 1:200, Cell signaling:), BRN2 (Rabbit, 1:200, Cell signaling:12137), GFAP (Chicken, 1:400, Abcam: ab4674), and SATB2 (Rabbit, 1:200, Invitrogen: PA5-83092).

Techniques: Generated, In Vitro, Immunostaining, Clone Assay, Marker, Staining, Expressing

Journal: Cell reports

Article Title: Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation

doi: 10.1016/j.celrep.2022.111942

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-CTIP2 , Abcam , Cat#: Ab28448; RRID: AB_1140055.

Techniques: Recombinant, Gentle, Knock-Out, Selection, Plasmid Preparation, Transfection, Protease Inhibitor, SYBR Green Assay, Bicinchoninic Acid Protein Assay, ATP Bioluminescent Assay, RNA Sequencing Assay, DNA Sequencing, Software, Real-time Polymerase Chain Reaction, Sequencing, Microscopy

Journal: Cell reports

Article Title: Mutations in the transcriptional regulator MeCP2 severely impact key cellular and molecular signatures of human astrocytes during maturation

doi: 10.1016/j.celrep.2022.111942

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Rabbit anti-CTIP2 , Abcam , Cat#: Ab28448; RRID: AB_1140055.

Techniques: Recombinant, Gentle, Knock-Out, Selection, Plasmid Preparation, Transfection, Protease Inhibitor, SYBR Green Assay, Bicinchoninic Acid Protein Assay, ATP Bioluminescent Assay, RNA Sequencing Assay, DNA Sequencing, Software, Real-time Polymerase Chain Reaction, Sequencing, Microscopy